How to Kill Mosquito Larvae in Standing Water Using BTI: A Complete Guide

How to Kill Mosquito Larvae in Standing Water Using BTI: A Complete Guide

Standing water is a mosquito's nursery. A single bottle cap can hold enough water for hundreds of larvae to develop into biting adults. If you want to stop mosquitoes at the source, the most effective and eco-friendly tool is Bacillus thuringiensis israelensis (BTI). This natural bacteria is a larvicide, meaning it specifically targets and kills mosquito larvae before they can take flight.

This guide will walk you through exactly how to use BTI to treat standing water on your property safely and effectively.

Why BTI is the Best Solution for Standing Water

BTI works through a precise biological action. It produces protein crystals that are toxic only to the larvae of mosquitoes, black flies, and fungus gnats. When mosquito larvae (often called "wrigglers") ingest these crystals, the toxins damage their gut lining, causing them to stop feeding and die within 24 to 48 hours.

The key advantages of BTI are:

  • Highly Specific & Safe: It is non-toxic to humans, pets, birds, fish, bees, butterflies, and beneficial insects. You can use it in ponds with wildlife.

  • Preventative: It breaks the mosquito life cycle at its most vulnerable stage, preventing new generations of biting adults.

  • Long-Lasting: Certain BTI products provide control for 30 days or more.

Step-by-Step: How to Apply BTI to Standing Water

Using BTI is straightforward. Your main choice is between two common product forms: Mosquito Dunks (donut-shaped) and Mosquito Bits (granular corn cob bits). Here’s how to use each.

1. Identify and Treat All Standing Water

First, scout your property for any water that has been stagnant for more than 4 days—the time it takes for eggs to hatch into larvae. Common breeding sites include:

  • Bird baths

  • Rain barrels and cisterns

  • Clogged gutters and drain pipes

  • Plant saucers and trays

  • Old tires, buckets, and trash can lids

  • Children's toys, tarps, and pool covers

  • Ponds and ornamental water features

  • Tree holes and hollow stumps

Tip: If you can simply tip over and eliminate the water (like in a bucket), do that first. Use BTI for water that must remain, like ponds or rain-collection barrels.

2. Choose Your BTI Product & Apply



For Long-Term Control (30+ Days) For Quick Knockdown & Small Areas
Product: Mosquito Dunks Product: Mosquito Bits
How to Use: Simply place one Dunk in any standing water body for every 100 square feet of surface area. It will float and slowly release BTI. How to Use: Sprinkle the granules evenly over the water's surface. Use about 1 teaspoon (or a small handful) per 25 square feet.
Best For: Rain barrels, ponds, animal watering troughs, unused pools, and any large, persistent water source. Best For: Small puddles, potted plant saucers, flooded areas, and damp soil. Also ideal for a rapid kill when you already see active wrigglers.

Application Tip for Flowing Water: In ditches or areas with slight water flow, use Mosquito Dunks but anchor them with a string or place them in a strategic spot where water pools to prevent them from washing away.

3. Reapply as Needed

  • Mosquito Dunks: Last for 30 days in most conditions. Mark your calendar to replace them monthly during warm breeding seasons.

  • Mosquito Bits: Provide quick control but are consumed faster. Reapply every 7 to 14 days for ongoing prevention.

Special Scenarios & Pro Tips

  • In Rain Barrels (for garden use): A Mosquito Dunk is perfect here. Place one in each barrel; it will not harm water quality for your plants. Check and replace monthly.

  • In Decorative Ponds with Fish: BTI is completely safe for fish and frogs. Use Dunks for easy, long-term control. Fish will often eat dead larvae, providing a double layer of defense.

  • In Hard-to-Reach Places: For clogged gutters or tree holes, Mosquito Bits can be tossed into the area. They will settle and release BTI where water collects.

  • For Large or Complex Properties: Consider a combination strategy. Use Bits for an immediate knockdown in problem areas, then install Dunks in all permanent water features for season-long prevention. Manufacturers also sell convenient combo packs.

Important Note: BTI only kills larvae in the water where it is applied. It has no effect on adult, flying mosquitoes. For complete control, pair your larvicide program with measures to kill adults (like traps) and protect yourself with repellents.

The Most Important Step: Consistency

The single biggest mistake is applying BTI once and forgetting about it. Mosquitoes are constantly laying new eggs. A consistent, repeat treatment schedule is what leads to a dramatic reduction in the local mosquito population over several weeks.

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